High-Throughput Methods For Analyzing Gene Activity Featured In Cold Spring Harbor Protocols
Our knowledge of epigenetics, the changes in gene regulation through modulation of chromatin not brought about by changes in the DNA sequence, has rapidly expanded in recent years. The first protocol describes a rapid, genome-wide method for identifying regions where methylation, an epigenetic control mechanism, is occurring. This method, "Methylated CpG Island Amplification and Microarray (MCAM) for High-Throughput Analysis of DNA Methylation," was developed by Marcos Estecio and Jean-Pierre Issa of the MD Anderson Cancer Center (http://www.mdanderson.org/) and Pearlly Yan and Tim Huang of the Ohio State University Comprehensive Cancer Center (http://www.jamesline.com/).
The protocol, freely accessible here, has proven successful for use in comparing normal tissues and tumors, helping researchers better understand the factors responsible for cancer.
The second protocol, available here, looks at the binding of regulatory proteins to DNA. These proteins play a role in turning activity of individual genes on and off. The method, "DNA Immunoprecipitation (DIP) for the Determination of DNA-binding Specificity," allows researchers to determine the specific DNA sequence that a regulatory protein binds. The technique allows for rapid screening of the entire genome for these binding sites, which gives insight into which genes these protein factors control.
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Article adapted by Start Sanatate from original press release.
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About Cold Spring Harbor Protocols:
Cold Spring Harbor Protocols (http://www.cshprotocols.org/) is a monthly peer-reviewed journal of methods used in a wide range of biology laboratories. It is structured to be highly interactive, with each protocol cross-linked to related methods, descriptive information panels, and illustrative material to maximize the total information available to investigators. Each protocol is clearly presented and designed for easy use at the bench - complete with reagents, equipment, and recipe lists. Life science researchers can access the entire collection via institutional site licenses, and can add their suggestions and comments to further refine the techniques.
About Cold Spring Harbor Laboratory Press:
Cold Spring Harbor Laboratory Press is an internationally renowned publisher of books, journals, and electronic media, located on Long Island, New York. Since 1933, it has furthered the advance and spread of scientific knowledge in all areas of genetics and molecular biology, including cancer biology, plant science, bioinformatics, and neurobiology. It is a division of Cold Spring Harbor Laboratory, an innovator in life science research and the education of scientists, students, and the public. For more information, visit http://www.cshlpress.com/.
Source: David Crotty
Cold Spring Harbor Laboratory
De mare cantitate de metode pentru analiza de gene de activitate prezentate în Cold Spring Harbor protocoale - High-Throughput Methods For Analyzing Gene Activity Featured In Cold Spring Harbor Protocols - articole medicale engleza - startsanatate