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454 Sequencing System Used To Determine The Complete Neanderthal Mitochondrial Genome, Establishing When Humans And Neanderthals Diverged

Researchers led by the Max-Planck Institute for Evolutionary Anthropology in Leipzig, Germany have published the complete Neanderthal mitochondrial genome sequence. The mitochondrial genome was generated using the 454 Sequencing technology to 35-fold redundancy, facilitating a detailed comparison against known human mitochondrial genomes and an analysis of recent human evolution. The study, entitled "A complete Neanderthal mitochondrial genome sequence determined by high-throughput sequencing" appears in the journal Cell.

The complete mitochondrial genome sequence was reconstructed from DNA obtained from a 38,000 year old Neanderthal bone fragment excavated in 1980 from the Vindija Cave, Croatia. Analysis of the assembled sequence unequivocally establishes that the Neanderthal mitochondrial DNA falls outside the variation of extant human mtDNAs, showing that Neanderthals did not contribute mtDNA to present-day humans. Furthermore, using the chimpanzee and human mitochondrial DNA sequences as reference points, the number of nucleotide differences found in the Neanderthal mitochondrial DNA establishes the divergence date between human and Neanderthal mtDNAs at 660,000±140,000 years.

"We are very excited to bring full scale genomic analysis to the study of our closest extinct relative. The mitochondrial result is only the tip of the iceberg as we push towards the completion of a whole Neanderthal genome sequence," said Dr. Svante Paabo, Ph.D. Senior Author on the paper and Director of the Department of Genetics at the Max Planck Institute for Evolutionary Anthropology.

The mitochondrial sequence data presented in the current study was generated as part of the Neanderthal Genome Project, an effort to sequence the entire nuclear genome of the Neanderthal. The mitochondrial sequencing is an important milestone towards the goal of whole Neanderthal sequencing as it demonstrates that ancient, degraded DNA can be sequenced accurately using the technology of the 454 Sequencing System and assembled into a consensus sequence. Given the mitochondrial genome's smaller size relative to the nuclear genome and their abundance in cells, where typically several hundred mtDNAs per nuclear genome exist, its sequencing was the logical first step in the full scale analysis of the Neanderthal genome.

The key deliverable for the Neanderthal Genome Project is to generate a Neanderthal genome sequence to be used as a reference point in the study of recent human evolution. The overarching purpose of the project is to identify areas in the genome where humans have undergone rapid evolution since the split from Neanderthals: the genetic changes that define us as human. This analysis is achieved by comparing the Neanderthal reference to the human and chimpanzee reference genome sequences. A significant result from the mtDNA genome sequence validates this approach: of the 13 proteins encoded in the mitochondrial DNA, one gene, subunit 2 of cytochrome c oxidase, is identified as having experienced a surprisingly large number of amino acid substitutions in modern humans since the split from Neanderthals. The project that was announced two years ago is expected to be completed later this year when a one-fold coverage of the Neanderthal genome is achieved.

"This exciting result from the Neanderthal Genome Project gives us a tantalizing look at the types of human evolutionary questions the community will be able to answer with a complete Neanderthal genome sequence" said Dr. Michael Egholm, Ph.D. Vice President of Research and Development at 454 Life Sciences. "Molecular anthropology is one of many fields revolutionized by the 454 Sequencing System's long highly-accurate reads, simple workflow, and ultra-high throughput."

454 Life Sciences, a center of excellence of Roche Applied Science, develops and commercializes the innovative 454 Sequencing System for ultra-high-throughput DNA sequencing. Specific applications include de novo sequencing and re-sequencing of genomes, metagenomics, RNA analysis, and targeted sequencing of DNA regions of interest. The hallmarks of the 454 Sequencing System are its simple, unbiased sample preparation and long, highly accurate sequence reads, including paired reads. The technology of the 454 Sequencing System has enabled many peer-reviewed studies in diverse research fields, such as cancer and infectious disease research, drug discovery, marine biology, anthropology, paleontology and many more.

For additional information, please visit http://www.454.com. For more information on the technology, visit http://www.roche-applied-science.com/sis/sequencing.

About Roche

Headquartered in Basel, Switzerland, Roche is one of the world's leading research-focused healthcare groups in the fields of pharmaceuticals and diagnostics. As the world's biggest biotech company and an innovator of products and services for the early detection, prevention, diagnosis and treatment of diseases, the Group contributes on a broad range of fronts to improving people's health and quality of life. Roche is the world leader in in-vitro diagnostics and drugs for cancer and transplantation, and is a market leader in virology. It is also active in other major therapeutic areas such as autoimmune diseases, inflammatory and metabolic disorders and diseases of the central nervous system. In 2007 sales by the Pharmaceuticals Division totalled 36.8 billion Swiss francs, and the Diagnostics Division posted sales of 9.3 billion francs. Roche has R&D agreements and strategic alliances with numerous partners, including majority ownership interests in Genentech and Chugai, and invested over 8 billion Swiss francs in R&D in 2007. Worldwide, the Group employs about 80,000 people. Additional information is available on the Internet at http://www.roche.com.

454, 454 SEQUENCING and 454 LIFE SCIENCES, are trademarks of 454 Life Sciences Corporation, Branford, CT, USA, a Roche company.

All other brands and product names are trademarks of their respective holders

http://www.roche.com





454 secvenþiere sistemul folosit pentru a determina complet Neandertal mitocondriale genom, de instituire Când oamenii ºi Neanderthals Diverged - 454 Sequencing System Used To Determine The Complete Neanderthal Mitochondrial Genome, Establishing When Humans And Neanderthals Diverged - articole medicale engleza - startsanatate